Human Immunoglobulin G (IgG) (Apt 8) Aptamer Details
ID# 7461
Structure
Chemistry
2'-F-RNA
Target
hlgG-Fc
Category
Protein
Affinity (Kd)
75 nM
Length
23
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprAprGprGpfUprGpfCpfUpfCpfCprGprAprAprAprGprGprAprApfCpfUpfCpfCp-3'
Molecular Weight: 7442.54
Extinction Coefficient: 226600
Binding Conditions / Buffer
Binding Buffer (145mM NaCl, 5.4 mM KCl, 0.8mM MgCl, 1.8 mM CaCl, and 20 mM Tris-HCl (pH 7.6)) Modifications at the 5' and 3' end have been made by the authors for various testing.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14,(2008): 1154-1163.
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