Aptagen

Human Colorectal Cancer WiDr Cell Line (HEW-K16) Aptamer Details

ID# 7582
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Structure
Human Colorectal Cancer WiDr Cell Line (HEW-K16) structure
Chemistry
Peptide
Target
Human Colorectal Cancer (WiDr Cell Line)
Category
Cells
Affinity (Kd)
N/A nM
Length
13
Aptazyme
No

Sequence (3-char table code)

G A C U/T
NH2-HisGluTrpSerTyrLeuAlaProTyrProTrpPheLys-COOH
Molecular Weight: 1878.16
Extinction Coefficient:

Binding Conditions / Buffer

107 cells/mL in PBS and 1.0% BSA with 1.5x1011 phages/mL
Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Rasmussen et al. "Tumor cell-targeting by phage-displayed peptides." Cancer Gene Therapy, 9(2002): 606-612
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