Huizenga ATP-Binding aptamer Aptamer Details
ID# 7993
Structure
Chemistry
DNA
Target
ATP
Category
Protein
Affinity (Kd)
956 µM
Length
36
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdApdCpdCpdTpdGpdGpdGpdGpdGpdApdGpdTpdApdTpdTpdGpdCpdGpdGpdApdGpdGpdApdApdGpdGpdTpdTpdCpdCpdApdGpdGpdTpdGp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Binding Temp: A Thiol-C6-modification is present at the 3' end to allow the aptamer to bind to the cargo molecule. See reference.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
V. C. Özalp, D. Çam, F. J. Hernandez, L. I. Hernandez, T. Schäfer, and H. A. Öktem, Small molecule detection by lateral flow strips via aptamer-gated silica nanoprobes, Analyst, 2016, 141, 2595.
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