HSM01 Aptamer Details

ID# 8235

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Structure

Chemistry

DNA

Target

Human Skeletal Muscle Cells

Sequence (3-char table code)

G A C U/T

5'-dApdCpdCpdGpdApdCpdCpdGpdTpdGpdCpdTpdGpdGpdApdCpdTpdCpdApdCpdCpdGpdGpdApdCpdApdApdApdApdCpdTpdTpdCpdApdGpdTpdTpdTpdTpdTpdApdTpdTpdTpdCpdCpdApdGpdApdTpdCpdCpdTpdGpdGpdGpdCpdApdTpdTpdGpdCpdGpdCpdCpdApdGpdGpdCpdTpdCpdGpdCpdTpdCpdApdTpdApdGpdTp-3'

Molecular Weight: 26400

Extinction Coefficient:

Binding Conditions / Buffer

Binding buffer: Dulbecco’s phosphate-buffered saline (D-PBS) supplemented with 4.5 g/L of glucose, 5 mmol/L of MgCl2, 0.1 mg/mL of yeast tRNA, and 1 mg/mL of BSA.

Binding Temp: 4°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Shuming Sun, Han Liu, Yan Hu, Yanpeng Wang, Mingri Zhao, Yijun Yuan, Yafei Han, Yingying Jing, Jin Cui, Xiaoxiang Ren, Xiao Chen, Jiacan Su, Selection and identification of a novel ssDNA aptamer targeting human skeletal muscle, Bioactive Materials, Volume 20, 2023, Pages 166-178, ISSN 2452-199X, https://doi.org/10.1016/j.bioactmat.2022.05.016. (https://www.sciencedirect.com/science/article/pii/S2452199X22002377)

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