HPV-HF cell line (14) Aptamer Details
ID# 7700
Structure
Chemistry
DNA
Target
HPV-nontumorigenic HF cell line
Category
Cells
Affinity (Kd)
7.1 nM
Length
89
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdAp dCpdCpdAp dGpdCpdTp dTpdApdTp dTpdCpdAp dApdTpdTp dGpdGpdGp dCpdGpdGp dGpdGpdAp dGpdTpdAp dGpdGpdGp dApdGpdAp dGpdGpdGp dGpdTpdTp dTpdCpdCp dApdTpdCp dGpdGpdCp dGpdApdCp dApdGpdAp dGpdGpdAp dGpdTpdTp dApdTpdGp dTpdGpdTp dGpdTpdAp dGpdApdTp dApdGpdTp dApdApdGp dTpdGpdCp dApdApdTp dCpdTp-3'
Molecular Weight: 27814.14
Extinction Coefficient: 893700
Binding Conditions / Buffer
1% BSA in PBS with salts
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Graham JC, Zarbl H (2012) Use of Cell-SELEX to Generate DNA Aptamers as Molecular Probes of HPV-Associated Cervical Cancer Cells. PLoS ONE 7(4): e36103. doi:10.1371/journal.pone.0036103
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