HMBA (aptabeacon) Aptamer Details
ID# 7716
Structure
Chemistry
DNA
Target
6X His-tag
Category
Protein
Affinity (Kd)
~4.2 nM
Length
42
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-{5'/5-FAM}-dCpdApdGpdGpdTpdTpdGpdGpdTpdCpdTpdGpdGpdTpdTpdGpdGpdGpdTpdTpdTpdGpdGpdCpdTpdCpdCpdTpdGpdTpdGpdTpdApdCpdGp-{Internal/Spacer 18}-{Internal/Spacer 18}-{Internal/Spacer 18}-{Internal/Spacer 18}-{Internal/Spacer 18}-{Internal/Spacer 18}-dCpdApdApdCpdCpdTpdGp-{3'/Dabcyl CPG}-3'
Molecular Weight: 12990.45
Extinction Coefficient: 382400
Binding Conditions / Buffer
1X DPBS without Ca and Mg (pH 7.1-7.5), 20 °C
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
X Tan et al. Molecular beacon aptamers for direct and universal quantitation of recombinant proteins from cell lysates. Anal. Chem. [published online ahead of print August 23, 2012]. doi: 10.1021/ac301764q
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