HIV-RT 3.17 Aptamer Details
ID# 8103
Structure
Chemistry
DNA
Target
HIV reverse transcriptase (HIV RT)
Category
Cells
Affinity (Kd)
0.38 nM
Length
50
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdTpdApdTpdGpdTpdApdGpdCpdApdTpdTpdTpdApdTpdGpdApdApdApdTpdTpdTpdTpdTpdApdApdApdTpdCpdApdApdTpdTpdTpdApdCpdTpdApdTpdTpdGpdGpdTpdGpdGpdTpdApdTpdCpdCp-3'
Molecular Weight: 15383.1
Extinction Coefficient: 491500
Binding Conditions / Buffer
20 mM Tris-HCl [pH 7.4], 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, and 1 mM CaCl2
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Dunn MR, McCloskey CM, Buckley P, Rhea K, Chaput JC. Generating Biologically Stable TNA Aptamers that Function with High Affinity and Thermal Stability. J Am Chem Soc. 2020 Apr 29;142(17):7721-7724. doi: 10.1021/jacs.0c00641. Epub 2020 Apr 20. PMID: 32298104.
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