HIV Reverse Transcriptase (PF1) Aptamer Details
ID# 7781
Structure
Chemistry
DNA
Target
HIV RT
Category
Protein
Affinity (Kd)
80 nM
Length
30
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdGpdApdApdGpdGpdCpdTpdTp dTpdApdGpdGpdTpdCpdTpdGpdApdGp dApdTpdCpdTpdCpdGpdGpdApdApdTp-3'
Molecular Weight: 9326.14
Extinction Coefficient: 300100
Binding Conditions / Buffer
50 mM Tris-HCl, pH 8, 1mM DTT, 80 mM KCl, 6 mM MgCl2 for ten minutes.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Y-T Lai and J DeStefano. DNA aptamers to human immunodeficiency virus reverse transcriptase selected by a primer-free SELEX method: characterization and comparison with other aptamers. Nucleic Acid Therapeutics 22(2012): 162-176.
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