HIV-1 Tat protein Aptamer Details
ID# 7730
Structure
Chemistry
RNA
Target
HIV-1 Tat protein
Category
Protein
Affinity (Kd)
120 pM
Length
37
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- rAprCprGprAprAprGprCprUprUprGp rAprUprCprCprCprGprUprUprUprGp rCprCprGprGprUprCprGprAprUprCp rGprCprUprUprCprGprAp-3'
Molecular Weight: 11784.08
Extinction Coefficient: 352600
Binding Conditions / Buffer
Binding Buffer: 50 mM Tris-HCl (pH 7.8) and 50 mM KCl
Binding Temp: r.t.°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
R Yamamoto et al. A novel RNA motif that binds efficiently and specifically to the Tat protein of HIV and inhibits the trans-activation by Tat of transcription in vitro and in vivo. Genes to Cells 5(2000):371-388
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