HIV-1 TAR RNA Hairpin Loop (B22-19) Aptamer Details
ID# 7473
Structure
Chemistry
DNA
Target
HIV-1 TAR RNA Hairpin Loop
Category
Small Organic
Affinity (Kd)
50 nM
Length
19
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdCpdCpdTpdApdGpdTpdTpdApdGpdCpdCpdApdTpdCpdTpdCpdCpdCp-3'
Molecular Weight: 5659.71
Extinction Coefficient: 166500
Binding Conditions / Buffer
R buffer (20 mM HEPES (pH 7.4), 140 mM potassium acetate, 20 mM sodium acetate, 3 mM magnesium acetate)
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Sekkai et al. "In Vitro Selection of DNA Aptamers Against the HIV-1 TAR RNA Hairpin." Antisense and Nucleic Acid Drug Development, 12 (2002): 265-274.
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