HIV-1 Reverse Transcriptase (R1T) Aptamer Details

ID# 7782

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Structure

Chemistry

DNA

Target

HIV-1 RT

Sequence (3-char table code)

G A C U/T

5'- dCpdGpdCpdCpdTpdGpdApdTpdTpdAp dGpdCpdGpdApdTpdApdCpdTpdCpdAp dGpdGpdCpdGpdTpdTpdGpdGpdGpdTp dGpdGpdGpdTpdGpdGpdGpdTpdGpdGp dGp-3'

Molecular Weight: 12825.34

Extinction Coefficient: 390800

Binding Conditions / Buffer

50 mM Tris-HCl, pH 8.0, 80 mM KCl, 6 mM MgCl2, and 1 mM DTT.

Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

D Michalowski et al. Novel bimodular DNA aptamers with guanosine quadruplexes inhibit phylogenetically diverse HIV-1 reverse transcriptases. Nucleic Acid Res. 36(2008): 7124-7135. Y-T Lai and J DeStefano. DNA aptamers to human immunodeficiency virus reverse transcriptase selected by a primer-free SELEX method: characterization and comparison with other aptamers. Nucleic Acid Therapeutics 22(2012): 162-176.

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