HIV-1 Reverse Transcriptase (4.3) Aptamer Details
ID# 7470
Structure
Chemistry
DNA
Target
HIV-1 RT
Category
Protein
Affinity (Kd)
190 pM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdGpdCpdApdGpdGpdTpdTpdTpdCpdGpdApdCpdGpdTpdApdCpdApdApdTpdGpdCpdTpdApdTpdGpdGpdApdGpdGpdCpdTpdTpdTpdApdTpdGpdApdTpdCpdGpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp-3'
Molecular Weight: 24799.14
Extinction Coefficient: 780100
Binding Conditions / Buffer
Binding buffer: 10 mM tris, 50 mM NaCl, and 1 mM EDTA (pH 7.5)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Mosing et al. "Capillary Electrophoresis-SELEX Selection of Aptamers with Affinity for HIV-1 Reverse Transcriptase." Analytical Chemistry, 77 (2005): 6107-6112.
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