HIV-1 Rev Peptide (L1-Rev) Aptamer Details

ID# 7778

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Structure

Chemistry

RNA

Target

HIV-1 Rev Peptide

Sequence (3-char table code)

G A C U/T

5'-rGprGprAprCprCprUprCprGprGprCprGprAprAprAprGprCprCprGprGprUprAprAprCprGprCprCprAprCprAprAprGprUprCprGprGprAprGprGprGprUprAprAprGprAprUprCprUprGprAprCprAprGprGprAprAprCprUprGprGprGprUprGprAprCprGprGprAprGprGprUprUprAprGprGprUprGprCp-3'

Molecular Weight: 25129.29

Extinction Coefficient: 774600

Binding Conditions / Buffer

Reaction buffer: 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 60 mM MgCl2

Binding Temp: 25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Hesslberth et al. "Simultaneous Detection of Diverse Analytes with an Aptazyme Ligase Array." Analytical Biochemistry, 312, (2003):106-112.

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