HIV-1 R5 SU Glycoprotein gp120 (B40t77) Aptamer Details
ID# 7732
Structure
Chemistry
2'-F-RNA
Target
HIV-1 R5 gp120
Category
Protein
Affinity (Kd)
31 nM
Length
77
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- rGprGprGprAprGprApfCprAprAprGp rApfCpfUprAprGprApfCprGpfCpfUp fCprAprApfUprGpfUprGprGprGpfCp fCprApfCprGpfCpfCpfCprGprApfUp fUpfUpfUprApfCprGpfCpfUpfUpfUp fUprApfCpfCpfCprGpfCprApfCprGp fCprGprApfUpfUprGprGpfUpfUpfUp rGpfUpfUpfUpfCpfCpfCp-3'
Molecular Weight: 24701.55
Extinction Coefficient: 737700
Binding Conditions / Buffer
cHBS buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM CaCl 2, 1 mM MgCl2, 2.7 mM KCl
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
A. K. Dey et. al. Structural characterization of an anti-gp120 RNA aptamer that neutralizes R5 strains of HIV-1. RNA 11:873-884
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