HIV-1 Nuceocapsid Aptamer Details
ID# 7537
Structure
Chemistry
RNA
Target
HIV-1 Nuceocapsid
Category
Protein
Affinity (Kd)
2.3 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprAprGprAprCprAprUprCprCprCprUprCprUprUprGprUprUprGprGprUprGprGprUprGprCprCprGprUprGprUprGprGprAprUprGprUprCprUprCp-3'
Molecular Weight: 12814.65
Extinction Coefficient: 381200
Binding Conditions / Buffer
Binding Buffer (50 mM Tris, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 30 µM ZnCl and 10 mM DTT) SSC buffer (17.5 mM sodium citrate (pH 7.0), 150 mM sodium chloride)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Berglund, A., et al. "A High Affinity Binding Site for the HIV-1 Nucleocapsid Protein." Nucleic Acids Research, 25(1997):1042-1049.
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