HIV-1 Integrase (P5) Aptamer Details
ID# 7534
Structure
Chemistry
RNA
Target
HIV-1 Integrase
Category
Protein
Affinity (Kd)
10 nM
Length
77
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprCprUprCprAprGprAprAprUprAprAprAprCprGprCprUprCprAprAprCprCprAprGprUprCprUprUprGprUprGprGprCprUprUprUprGprAprAprAprGprAprGprAprGprGprAprGprUprGprUprUprCprGprAprCprAprUprGprAprGprGprCprCprCprGprGprAprUprCprCprGprGprCp-3'
Molecular Weight: 24933.1
Extinction Coefficient: 767300
Binding Conditions / Buffer
Buffer A (50 mM HEPES, 1 mM DTT, 1 mM APMSF) Binding Buffer (50 mM Na-HEPES (pH 7.5), 250 mM NaCl, 2 mM DTT, 10 mM MnCl2, 5 mM CHAPS)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Allen, P., et al. "Isolation of High-Affinity RNA Ligands to HIV-1 Integrase from a Random Pool." Virology, 209(1995): 327-336.
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