Histone (H4K16Ac) Aptamer Details
ID# 7722
Structure
Chemistry
DNA
Target
H4K16Ac
Category
Peptide
Affinity (Kd)
47 nM
Length
48
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdApdCpdGpdTpdApdApdGpdTpdTpdApdApdTpdTpdGpdGpdApdCpdTpdTpdGpdGpdTpdCpdGpdTpdGpdTpdGpdCpdGpdGpdCpdApdCpdApdGpdCpdGpdApdTpdTpdGpdApdApdApdTp-3'
Molecular Weight: 14926.78
Extinction Coefficient: 476300
Binding Conditions / Buffer
Washed with buffer A (150 mM NaCl, 5 mM Na2HPO4, 1 mM EDTA, pH 7.5) and PBS buffer (150 mM NaCl, 5 mM Na2HPO4. pH 7.5) three times.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Lin et al. "Recognition Imaging of Acetylated Chromatin Using a DNA Aptamer." Biophysical Journal, 97(2009): 1804-07.
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