Histone H4 N-terminal tail (4.33) Aptamer Details
ID# 7721
Structure
Chemistry
DNA
Target
H4 N-terminal tail
Category
Protein
Affinity (Kd)
1.3 nM
Length
50
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdGpdGpdTpdGpdGpdGpdGpdTpdTpdCpdCpdCpdGpdGpdGpdApdGpdGpdGpdCpdGpdGpdCpdTpdApdCpdGpdGpdGpdTpdTpdCpdCpdGpdTpdApdApdTpdCpdApdGpdApdTpdTpdTpdGpdTpdGpdTp-3'
Molecular Weight: 15592.13
Extinction Coefficient: 473300
Binding Conditions / Buffer
Low-salt conditions: 100 mM NaCl, 5 mM MgCl2, and 10 mM HEPES (pH 7.5).
High-salt conditions: 500 mM NaCl, 10 mM MgCl2, and 10 mM HEPES (pH 7.5).
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yu et al. "Aptamers can Discriminate Alkaline Proteins with High Specificity." Chem. Biochem. , 12(2011): 2659-2666.
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