HER-2 (S6) Aptamer Details
ID# 7670
Structure
Chemistry
RNA
Target
HER-2 (SK-BR-3 cell line)
Category
Cells
Affinity (Kd)
94.6 nM
Length
98
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprUprAprCprCprAprGprCprUprUprAprUprUprCprAprAprUprUprUprGprGprAprUprGprGprGprGprAprGprAprUprCprCprGprUprUprGprAprGprUprAprAprGprCprGprGprGprCprGprUprGprUprCprUprCprUprCprUprGprCprCprGprCprCprUprUprGprCprUprAprUprGprGprGprGprAprGprAprUprAprGprUprAprAprGprUprGprCprAprAprUprCprUp-3'
Molecular Weight: 31658
Extinction Coefficient: 976700
Binding Conditions / Buffer
Binding buffer: 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, 1 mg/mL BSA in Dulbeccoƒ??s PBS.
Washing buffer: 4.5 g/L glucose, 5 mM MgCl2 in Dulbeccoƒ??s PBS
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kang, H. (2009). Isolation of RNA aptamers targeting Her-2-overexpressing breast cancer cells using cell-selex. Bull. Korean Chem. Soc., 30(8), 182-1831.
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