Hepatitis C Virus Non-Structural Protein 3 (G9-1) Aptamer Details

ID# 7529

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Structure

Chemistry

RNA

Target

Hepatitis C Virus Non-Structural Protein 3

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprAprGprAprAprUprUprCprCprGprAprCprCprAprGprAprAprGprCprUprUprCprGprGprGprAprUprUprUprGprAprGprGprGprUprAprGprAprAprUprGprGprGprAprCprUprAprCprCprUprUprUprCprCprUprCprUprCprUprCprCprUprUprCprCprUprCprUprUprCprUp-3'

Molecular Weight: 23294.92

Extinction Coefficient: 704000

Binding Conditions / Buffer

Binding Buffer (50 mM Tris/HCl (pH 7.8), 30 mM NaCl, 5 mM CaCl2 and 10 mM dithiothreitol) Elution Buffer (0.4 m sodium acetate, 5 mm EDTA and 7 m urea (pH 5.5))

Binding Temp:

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Fukuda, et al. "Isolation and characterization of RNA aptamers specific for the hepatitis C virus nonstructural protein 3 protease." European Journal of Biochemistry, 267(2000): 3685-3694.

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