Hepatitis C NS3 Protein (10G-1) Aptamer Details

ID# 7535

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Structure

Chemistry

RNA

Target

Hepatitis C NS3 Protein

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprAprGprAprGprCprGprGprAprAprGprCprGprUprGprCprUprGprGprGprCprCp rAprGprUprAprGprUprGprUprAprUprAprGprGprGprCprUprCprGprAprAprAprUprGprUprUprCprAprUprGprGprCprUprCprAprGprUprGprGprAp rCprAprUp -3'

Molecular Weight: 21495.01

Extinction Coefficient: 665000

Binding Conditions / Buffer

Binding Buffer: 50 mM Tris/HCl (pH 7.7), 30 mM NaCl, 5 mM CaCl2, 10 mM dithiothreitol and 1% BSA Elution Buffer (200 uL of 0.4 M sodium acetate, 5 mM EDTA, and 7 M urea (pH 5.5))

Binding Temp: 23°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Urvil, P., et al. "Selection of RNA aptamers that bind specifically to the NS3 protease of hepatitis C virus." European Journal of Biochemistry, 248(1997):130-138.

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