Hepatitis B Virus (HBV) Polymerase (P protein) (A9) Aptamer Details
ID# 7644
Structure
Chemistry
RNA
Target
Hepatitis B Virus (HBV) Polymerase (P protein)
Category
Protein
Affinity (Kd)
N/A nM
Length
61
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- rUprGprUprUprCprAprUprGprUprCp rCprUprAprCprUprGprUprUprCprAp rAprAprCprAprAprAprAprAprAprAp rCprUprGprUprGprCprAprCprAprAp rAprAprAprUprAprAprAprUprUprGp rGprGprGprCprAprUprGprGprAprCp rAp-3'
Molecular Weight: 19585.92
Extinction Coefficient: 626700
Binding Conditions / Buffer
binding buffer (0.1 M sodium phosphate (pH 7.4), 150 mM NaCl, 20 mM imidazol, 0.1% (v/v) NP-40, 100 mg/ml yeast tRNA), 30 °C
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Feng et al. "A SELEX-Screened Aptamer of Human Hepatitis B Virus RNA Encapsidation Signal Suppresses Viral Replication." PLoS One, 6(2011): e27862
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