HD1-60.29-15dA Aptamer Details
ID# 8223
Structure
Chemistry
DNA
Target
Thrombin
Category
Protein
Affinity (Kd)
4.9 nM
Length
59
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGpdApdApdApdApdApdApdApdApdApdApdApdApdApdApdApdApdGpdTpdCpdCpdGpdTpdGpdGpdTpdApdGpdGpdGpdCpdApdGpdGpdTpdTpdGpdGpdGpdGpdTpdGpdApdCpdTp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
1x PBS, 3mM Magnesium Chloride. 1 ug/uL BSA.
Binding Temp: 20°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Müller J, Wulffen B, Pötzsch B, Mayer G. Multidomain targeting generates a high-affinity thrombin-inhibiting bivalent aptamer. Chembiochem. 2007 Dec 17;8(18):2223-6. doi: 10.1002/cbic.200700535. PMID: 17990265.
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