HA Clone B Aptamer Details

ID# 8182

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Structure

Chemistry

RNA

Target

Hemagglutinin of H3N2

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprAprGprAprAprUprUprCprCprGprAprCprCprAprGprAprAprGprGprGprUprUprAprGprCprGprGprUprCprGprUprCprUprUprAprAprGprUprAprGprUprUprUprUprUprGprGprUprCprCprUprUprUprCprCprUprCprUprCprUprCprCprUprUprCprCprUprCprUprUprCprUp-3'

Molecular Weight: 22308.9

Extinction Coefficient: 642000

Binding Conditions / Buffer

0.01 M Hepes (pH 7.4), 0.15 M NaCl, 3 mM EDTA, and 0.005% Tween 20

Binding Temp: 22°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Misono, T.S. and Kumar, P.K., 2005. Selection of RNA aptamers against human influenza virus hemagglutinin using surface plasmon resonance. Analytical biochemistry, 342(2), pp.312-317.

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