H6 Aptamer Details
ID# 8140
Structure
Chemistry
DNA
Target
Triclosan
Category
Protein
Affinity (Kd)
369.82 nM
Length
75
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5′-dApdTpdApdCpdGpdApdGpdCpdTpdTpdGpdTpdTpdCpdApdApdTpdApdGpdTpdCpdTpdGpdApdApdGpdGpdGpdApdTpdTpdGpdApdGpdTpdApdTpdCpdCpdGpdGpdGpdGpdApdGpdApdTpdGpdApdTpdGpdTpdCpdGpdTpdTpdGpdGpdTpdGpdApdTpdApdGpdTpdApdApdGpdApdGpdCpdApdApdTpdCp-3′
Molecular Weight: 23407.2
Extinction Coefficient: 758500
Binding Conditions / Buffer
100 mM NaCl, 2 mM KCl, 5 mM MgCl2,
1 mM CaCl2, and 20 mM Tris-HCl, pH 7.5
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Li S, Clarkson M, McNatty K. Selection and characterisation of triclosan-specific aptamers using a fluorescence microscope-imaging assay. Anal Bioanal Chem. 2020 Oct;412(26):7285-7294. doi: 10.1007/s00216-020-02863-7. Epub 2020 Aug 11. PMID: 32780154.
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