H2 Aptamer Details
ID# 8226
Structure
Chemistry
DNA
Target
HER-2
Category
Protein
Affinity (Kd)
270 nM
Length
54
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdCpdCpdGpdTpdCpdGpdApdApdCpdApdCpdGpdApdGpdCpdApdTpdGpdGpdTpdGpdCpdGpdTpdGpdGpdApdCpdCpdTpdApdGpdGpdApdTpdGpdApdCpdCpdTpdGpdApdGpdTpdApdCpdTpdGpdTpdCpdCp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
Binding buffer: 100 mM NaCl, 20 mM Tris–HCl, pH 7.6, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween 20
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
"In vitro HER2 protein-induced affinity dissociation of carbon nanotube-wrapped anti-HER2 aptamers for HER2 protein detection" J. H. Niazi, S. K. Verma, S. Niazi and A. Qureshi, Analyst, 2014
DOI: https://doi.org/10.1039/C4AN01665C
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