H1 Aptamer Details
ID# 8086
Structure
Chemistry
DNA
Target
Poly(histidine) tag (His-tag)
Category
Protein
Affinity (Kd)
86 nM
Length
79
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dApdTpdApdCpdGpdApdCpdTpdCpdApdCpdTpdApdTpdTpdApdGpdGpdGpdApdTpdGpdTpdCpdCpdApdCpdCpdApdTpdApdTpdApdGpdApdTpdCpdGpdApdTpdTpdTpdApdApdGpdTpdCpdCpdCpdTpdCpdGpdTpdTpdApdTpdTpdApdApdTpdGpdCpdTpdApdGpdApdCpdTpdApdCpdTpdGpdApdCpdTpdApdCpdApdAp-3’
Molecular Weight: 24224.8
Extinction Coefficient: 777800
Binding Conditions / Buffer
20 mM Tris-HCl, 100 mM NaCl, 5 mM MgCl2, and 0.1% bovine
serum albumin with 10µg/mL yeast tRNA and 10µg/mL salmon sperm DNA
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Wang Y, Li Z, Yu H. Aptamer-Based Western Blot for Selective Protein Recognition. Front Chem. 2020 Oct 29;8:570528. doi: 10.3389/fchem.2020.570528. PMID: 33195056; PMCID: PMC7658645.
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