H02 Aptamer Details

ID# 7999

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Structure

Chemistry

RNA

Target

Cells expressing Integrin a5B1 & Recombinant a5B1 Integrin

Sequence (3-char table code)

G A C U/T

5’-dGpdGpdUp dUpdApdCp dCpdApdGp dCpdCpdUp dUpdCpdAp dCpdUpdGp dCpdGpdGp dApdCpdGp dGpdApdCp dApdGpdAp dGpdApdGp dUpdGpdCp dApdApdCp dCpdUpdGp dCpdCpdGp dUpdGpdCp dCpdGpdCp dApdCpdCp dApdCpdGp dGpdUpdCp dGpdGpdUp dCpdApdCp dApdCp-3’

Molecular Weight: 20749.2

Extinction Coefficient: 620700

Binding Conditions / Buffer

Selection Buffer: 1 mM MgCl2 & 0.5 mM CaCl2 in PBS (pH 7.4)

Binding Temp: Denature at 80°C for 2 min, immediately cool on ice for 5 min, and then keep at room temperature in selection buffer.

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Fechter, P.; Cruz Da Silva, E.; Mercier, M.C.; Noulet, F.; Etienne-Seloum, N.; Guenot, D.; Lehmann, M.; Vauchelles, R.; Martin, S.; Lelong-Rebel, I.; Ray, A.M.; Seguin, C.; Dontenwill, M.; Choulier, L. RNA Aptamers Targeting Integrin a5B1 as Probes for Cyto- and Histofluorescence in Glioblastoma. Mol. Ther. –Nucleic Acids. 2019, 17, 63-77.

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