H Seq02 against Sterigmatocystin Aptamer Details

ID# 9187

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Structure

Chemistry

DNA

Target

Sterigmatocystin (ST) - water-insoluble small-molecule targe

Sequence (3-char table code)

G A C U/T

5'-dGpdApdApdTpdApdCpdGpdGpdApdTpdTpdApdCpdTpdGpdTpdTpdCpdGpdTpdApdCpdTpdGpdApdCpdCpdTpdApdTpdGpdCpdTpdGpdTpdGpdGpdApdGpdTp-3'

Molecular Weight: 12357 g/mole

Extinction Coefficient: 387200

Binding Conditions / Buffer

Molecular Activation of ST: Glycolic acid (C2H4O3, 1 g) dissolved in 4 mL of trifluoroacetic acid (C2HF3O2), 4 mL of 2.5 mg/mL ST. Capture SELEX: 200ul of Dulbecco’s phosphate-buffered saline (DPBS), pH of 7.4 Aptamer Affinity Column: 1 mL of blocking solution (0.2% glycine solution, pH of 6.0), 1 mL of DPBS, 1 mL of PBST, and 1 mL of water.

Binding Temp: 25 °C°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Zheng, M., Ye, J., Liu, H., Wu, Y., Shi, Y., Xie, Y., & Wang, S. (2024). FAM Tag Size Separation-Based Capture-Systematic Evolution of Ligands by Exponential Enrichment for Sterigmatocystin-Binding Aptamers with High Specificity. Analytical chemistry, 96(2), 710–720. https://doi.org/10.1021/acs.analchem.3c03675.

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