Aptagen

h-Ras Aptamer Details

ID# 8184
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Structure
h-Ras structure
Chemistry
DNA
Target
h-Ras protein
Category
Protein
Affinity (Kd)
200 nM
Length
80
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dCpdTpdTpdCpdTpdGpdCpdCpdCpdGpdCpdCpdTpdCpdCpdTpdTpdCpdCpdTpdApdTpdTpdApdGpdGpdGpdTpdGpdCpdTpdTpdCpdGpdCpdGpdApdApdGpdTpdTpdCpdApdTpdTpdTpdTpdApdCpdApdTpdCpdCpdCpdCpdApdTpdApdGpdGpdApdGpdApdCpdGpdApdGpdApdTpdApdGpdGpdCpdGpdGpdApdCpdApdCpdTp-3'
Molecular Weight: 24507.9
Extinction Coefficient: 741700

Binding Conditions / Buffer

50 mM Tris-HCl at pH 8.2
Binding Temp: 20°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Berezovski, M., Musheev. M., Drabovich, A., and S. N. Krylov. 2006. Non-SELEX Selection of Aptamers. Journal of the American Chemical Society. 128, 1410-1411. doi: 0.1021/ja056943j
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