GVI-7 Aptamer Details
ID# 8083
Structure
Chemistry
DNA
Target
GCRV-infected CIK
Category
Cells
Affinity (Kd)
176.63 nM
Length
90
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdTpdCpdTpdGpdApdApdGpdTpdApdGpdApdCpdGpdCpdApdGpdGpdApdGpdTpdGpdApdApdCpdCpdCpdApdCpdCpdTpdCpdApdGpdGpdGpdCpdApdTpdCpdTpdTpdApdCpdApdTpdTpdTpdCpdTpdTpdCpdTpdApdApdGpdTpdTpdGpdTpdTpdApdCpdCpdApdTpdGpdTpdTpdTpdApdGpdTpdCpdApdCpdApdCpdCpdTpdGpdApdGpdTpdApdApdGpdCpdGpdTp-3'
Molecular Weight: 27920.1
Extinction Coefficient: 874000
Binding Conditions / Buffer
Binding Buffer [10% FBS] from Life Technologies.
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yu, Qing et al. “Generation and characterization of aptamers against grass carp reovirus infection for the development of rapid detection assay.” Journal of fish diseases vol. 44,1 (2021): 33-44. doi:10.1111/jfd.13265
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