Gonadotropin-releasing hormone 1 (60-mer S42) Aptamer Details
ID# 7493
Structure
Chemistry
L-DNA
Target
Gonadoliberin/Gonadotropin-releasing hormone 1 (GnRH)
Category
Peptide
Affinity (Kd)
45 nM
Length
60
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-LdGLdCLdGLdGLdCLdGLdGLdALdGLdGLdGLdTLdGLdGLdGLdCLdTLdGLdGLdGLdGLdCLdTLdGLdGLdGLdCLdCLdGLdGLdGLdGLdGLdGLdCLdGLdTLdGLdCLdGLdTLdALdALdGLdCLdALdCLdGLdTLdALdGLdCLdCLdTLdCLdGLdCLdCLdGLdC-3'
Molecular Weight: 48667.48
Extinction Coefficient: 555200
Binding Conditions / Buffer
Selection Buffer: 10 mM HEPES (pH 7.5), 137 mM NaCl, 2.7 mM KCl, 2 mM CaCl22, 1 mM MgCl2, 0.005% Triton X-100
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Leva, S., et al. "GnRH Binding RNA and DNA Spiegelmers: A Novel Approach toward GnRH Antagonism." Chemistry and Biology, 9 (2002): 351-359.
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