GL21.T Aptamer Details
ID# 9015
Structure
Chemistry
RNA
Target
Axl
Category
Protein
Affinity (Kd)
12 nM
Length
34
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
rAprUprGprAprUprCprAprAprUprCprGprCprCprUprCprAprAprUprUprCprGprAprCprAprGprGprAprGprGprCprUprCprAprCp
Molecular Weight: 10297.6 g/mole
Extinction Coefficient: 319300
Binding Conditions / Buffer
filter binding analysis with the soluble extracellular domain of human Axl, Dtk, and Mer as targets (R&D Systems, Minneapolis, MN), was performed by incubating 1 nmol/l of radiolabeled aptamers with 1, 3.2, 10, 32, 100, 320, and 1,000 nmol/l of EC-Axl, EC-Dtk, or EC-Mer
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Cerchia L, Esposito CL, Camorani S, Rienzo A, Stasio L, Insabato L, Affuso A, de Franciscis V. Targeting Axl with an high-affinity inhibitory aptamer. Mol Ther. 2012 Dec;20(12):2291-303. doi: 10.1038/mt.2012.163. Epub 2012 Aug 21. PMID: 22910292; PMCID: PMC3519989.
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