GHS-R binding peptide (G5-1) Aptamer Details
ID# 7713
Structure
Chemistry
Peptide
Target
growth hormone secretagogue receptor
Category
Protein
Affinity (Kd)
2.4 nM
Length
28
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-PheGlnPheLeuProPheMetPheHisGlnArgValGlnGlnArgLysGluSerLysLysProProAlaLysLeuGlnProArg-3'
Molecular Weight: 3922.6
Extinction Coefficient:
Binding Conditions / Buffer
10mM Hepes-Na-OH, pH 7.4: 135 mM NaCl, 5 mM KCl, 2.5 mM CaCl2, 0.8 mM MgCl2, 10 mM glucose, 0.2% BSA, and 0.6 mM NaHCO3. 4 °C.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
S Ueno et al. In vitro selection of a peptide antagonist of growth hormone secretagogue receptor using cDNA display. PNAS, posted on June 20, 2012, doi: 10.1073/pnas.1203561109 (to appear in print)
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