Gentamycin (LS13) Aptamer Details
ID# 7799
Structure
Chemistry
2'-F-RNA
Target
Gentamycin and other Aminoglycosides (AMGs)
Category
Small Organic
Affinity (Kd)
26.7 nM
Length
79
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGp rAprGprAp rAprGprGp fCprGprGp fCprGpfCp rGpfUprAp rGprGpfCp rGprAprGp fCpfUpfUp fUprApfCp fCprAprGp fUpfUpfUp fUprApfUp fUpfUprGp fUpfUpfUp fUprApfUp fUprGpfUp fUprApfUp rApfUprGp fCpfUpfUp rApfUpfUp fCprGpfUp rGprGprAp fCprGprAp fUprGpfCp rGpfCprAp fCp-3'
Molecular Weight: 25474.89
Extinction Coefficient: 788000
Binding Conditions / Buffer
Binding Buffer: PBS with 10% (v/v) methanol
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
N. Derbyshire et al. Toggled RNA aptamers against aminoglycosides allowing facile detection of antibiotics using gold nanoparticle assays. Anal. Chem. (2012) 84: 6595-6602.
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