Gelatinase (MMP-9) Aptamer Details
ID# 7625
Structure
Chemistry
Peptide
Target
Matrix Metalloproteinase-9 (MMP-9)
Category
Protein
Affinity (Kd)
N/A nM
Length
10
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
NH2-CysThrThrHisTrpGlyPheThrLeuCys-COOH
Molecular Weight: 1268.54
Extinction Coefficient:
Binding Conditions / Buffer
Incubated overnight at 4 ° C in binding buffer (50 mM TBS buffer (Tris-HCl/0.1 M NaCl, pH 7.5) containing 1% BSA).
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Koivunen et al. "Tumor targeting with a selective gelatinase inhibitor." Nature Biotechnology, 17(1999): 768 - 774.
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