GBM128 Aptamer Details
ID# 7943
Structure
Chemistry
DNA
Target
Glioblastoma
Category
Cells
Affinity (Kd)
20 nM
Length
83
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dGpdApdAp dTpdTpdCp dApdGpdTp dCpdGpdGp dApdCpdAp dGpdCpdGp dApdCpdGp dGpdTpdGp dGpdGpdAp dGpdCpdCp dCpdCpdAp dApdApdTp dApdApdTp dTpdCpdTp dTpdGpdCp dGpdApdTp dTpdApdTp dTpdApdGp dTpdGpdTp dApdApdGp dCpdGpdGp dApdTpdGp dGpdApdCp dGpdApdAp dTpdApdTp dCpdGpdTp dCpdTpdCp dCpdCp
Molecular Weight: 25689.73
Extinction Coefficient: 812600
Binding Conditions / Buffer
The washing buffer (WB) used contained 4.5 g/L glucose, and 5 mM MgCl2 in PBS (pH = 7.4). The binding buffer used during selection was prepared by adding yeast tRNA (0.1 mg/mL) and BSA (1 mg/mL) to washing buffer to reduce background binding.
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kang D, Wang J, Zhang W, Song Y, Li X, et al. (2012) Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity. PLoS ONE 7(10): e42731. doi:10.1371/journal.pone.0042731
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