G12msi Aptamer Details
ID# 8073
Structure
Chemistry
DNA
Target
GPC3
Category
Protein
Affinity (Kd)
0.17 nM
Length
37
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdTpdGpdApdApdTpdTpdGpdCpdGpdTpdTpdGpdApdApdTpdTpdApdTpdGpdTpdDpdDpdDpdTpdTpdCpdApdGpdTpdCpdApdTpdCpdApdCp dTp -3'
Molecular Weight: 11399.4
Extinction Coefficient: 358601.769336
Binding Conditions / Buffer
4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, and 1 mg/mL
bovine serum albumin
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Park, J. Y., Chae, J. R., Cho, Y. L., Kim, Y., Lee, D., Lee, J. K., & Kang, W. J. (2020). Targeted Therapy of Hepatocellular Carcinoma Using Gemcitabine-Incorporated GPC3 Aptamer. Pharmaceutics, 12(10), 985. doi:10.3390/pharmaceutics12100985
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