Aptagen

FX2 Aptamer Details

ID# 9436
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Structure
FX2 structure
Chemistry
DNA
Target
Flunixin
Category
Small Organic
Affinity (Kd)
25600 nM
Length
45
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dGpdGpdGpdApdCpdGpdApdCpdTpdGpdGpdGpdGpdGpdTpdApdGpdGpdTpdApdApdGpdGpdTpdTpdGpdGpdTpdTpdCpdTpdTpdGpdTpdGpdApdGpdGpdTpdCpdGpdTpdCpdCpdCp-3'
Molecular Weight: 14076.1 g/mole
Extinction Coefficient: 434100

Binding Conditions / Buffer

78.6°C
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Obtin Alkhamis, Caleb Byrd, Juan Canoura, Adara Bacon, Ransom Hill, Yi Xiao, Exploring the relationship between aptamer binding thermodynamics, affinity, and specificity, Nucleic Acids Research, Volume 53, Issue 6, 11 April 2025, gkaf219, https://doi.org/10.1093/nar/gkaf219
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