Aptagen

FX1 Aptamer Details

ID# 8802
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Structure
FX1 structure
Chemistry
DNA
Target
Flunixin
Category
Small Organic
Affinity (Kd)
0.28±0.02 µM
Length
47
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dGpdGpdGpdApdCpdGpdApdCpdGpdGpdCpdCpdCpdCpdTpdTpdTpdTpdApdApdGpdGpdApdGpdGpdTpdApdGpdApdGpdGpdTpdGpdTpdTpdCpdGpdCpdTpdGpdTpdCpdGpdTpdCpdCpdCp-3'
Molecular Weight: 14543.4
Extinction Coefficient: 442500

Binding Conditions / Buffer

PBS (pH 7.4)- 8.1 mM Na2HPO4, 1.5 mM KH2PO4, 138 mM NaCl, 2.7 mM KCl, & 1 mM MgCl2
Binding Temp: ~20°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Alkhamis O. & Xiao Y. (2022). Systematic Study of in Vitro Selection Stringency Reveals How To Enrich High-Affinity Aptamers. Journal of the American Chemical Society, 145(1) https://doi.org/10.1021/jacs.2c09522
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