Aptagen

Furaneol (Fur_14) Aptamer Details

ID# 10066
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Structure
Furaneol (Fur_14) structure
Chemistry
DNA
Target
Furaneol
Category
Small Organic
Affinity (Kd)
1.1 ± 0.4 µM
Length
93
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dCpdGpdApdCpdCpdApdGpdCpdTpdCpdApdTpdTpdCpdCpdTpdCpdApdCpdCpdApdCpdGpdApdGpdApdApdApdGpdGpdApdGpdCpdTpdCpdGpdApdTpdGpdApdApdCpdTpdGpdCpdGpdApdCpdGpdGpdApdTpdTpdCpdGpdApdCpdCpdCpdTpdApdTpdGpdCpdGpdApdGpdTpdApdGpdGpdTpdGpdGpdTpdGpdGpdApdTpdCpdCpdGpdApdGpdCpdTpdCpdApdCpdCpdApdGpdTp-3'
Molecular Weight: 28680.6 g/mole
Extinction Coefficient: 891600

Binding Conditions / Buffer

100 mM NaCl, 20 mM Tris-HCl [pH 7.6], 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Komarova, N.; Andrianova, M.; Glukhov, S.; Kuznetsov, A. Selection, Characterization, and Application of ssDNA Aptamer against Furaneol. Molecules 2018, 23, 3159. https://doi.org/10.3390/molecules23123159
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