FUB5 Aptamer Details
ID# 9446
Structure
Chemistry
DNA
Target
Flunixin
Category
Small Organic
Affinity (Kd)
163 nM
Length
46
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdApdCpdGpdApdCpdGpdGpdApdGpdGpdTpdGpdTpdApdGpdGpdGpdTpdTpdTpdApdGpdGpdTpdApdGpdTpdGpdGpdTpdTpdTpdGpdApdApdGpdTpdCpdGpdTpdCpdCpdCp-3'
Molecular Weight: 14413.3 g/mole
Extinction Coefficient: 455300
Binding Conditions / Buffer
77.9°C
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Obtin Alkhamis, Caleb Byrd, Juan Canoura, Adara Bacon, Ransom Hill, Yi Xiao, Exploring the relationship between aptamer binding thermodynamics, affinity, and specificity, Nucleic Acids Research, Volume 53, Issue 6, 11 April 2025, gkaf219, https://doi.org/10.1093/nar/gkaf219
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