Florfenicol (Apt-14t) Aptamer Details

ID# 10038

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Structure

Chemistry

DNA

Target

Florfenicol

Sequence (3-char table code)

G A C U/T

5'-dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdApdCpdApdGpdTpdApdGpdTpdGpdApdGpdGpdGpdGpdTpdCpdCpdGpdTpdCpdGpdTpdGpdGpdGpdGpdTpdApdGpdTpdTpdGpdGpdGpdTpdCpdGpdTpdGpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'

Molecular Weight: 23679.3 g/mole

Extinction Coefficient: 755800

Binding Conditions / Buffer

100 mM NaCl, 20 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween 20, pH 7.6

Binding Temp: Room temperature°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Shi, M.; Liu, R.; Zhang, F.; Chitrakar, B.; Wang, X. Screening of Single-Stranded DNA Aptamer Specific for Florfenicol and Application in Detection of Food Safety. Biosensors 2022, 12, 701. https://doi.org/10.3390/bios12090701

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