Flavin Mononucleotide (AR5) Aptamer Details
ID# 7599
Structure
Chemistry
RNA
Target
FMN
Category
Small Organic
Affinity (Kd)
N/A nM
Length
88
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprAprUprAprAprUprAprGprCprCprGprUprAprGprGprUprUprGprCprGprAprAprAprGprCprGprAprCprCprCprUprGprAprUprGprAprGp rCprCprUprUprAprGprGprAprUprAprUprGprCprAprUprGprAprUprGprCprAprGprAprAprGprGprAprCprGprUp rCprGprAprAprAprCprGprGprUprAprGprCprGprAprGprAprGprCprUprCp -3'
Molecular Weight: 28595.37
Extinction Coefficient: 897000
Binding Conditions / Buffer
Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl2
2X Gel Loading Buffer (equal volume used to quench reaction): 87 mM Tris base, 89 mM boric acid, 20% sucrose, 0.05% bromophenol blue, 0.05% xylene cyanol, 0.1% sodium dodecyl sulfate (SDS), 7.3 M urea, 1 mM EDTA. Additional 40 mM EDTA added.
Binding Temp: 23°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Seetharaman et al. "Immobilized RNA switches for the analysis of complex chemical and biological mixtures." Nature Biotechnology, 19(2001): 336-341.
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