Fibrinogen (85A/Ap90) Aptamer Details
ID# 7609
Structure
Chemistry
DNA
Target
Fibrinogen
Category
Protein
Affinity (Kd)
63.8 nM
Length
90
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdCpdTpdTpdCpdGpdTpdTpdGpdTpdCpdTpdGpdCpdCpdTpdTpdCpdGpdTpdApdGpdGpdApdCpdCpdGpdCpdApdGpdApdCpdApdTpdCpdGpdApdCpdGpdCpdApdGpdGpdGpdApdApdApdTpdTpdCpdCpdGpdCpdApdApdGpdTpdCpdCpdApdGpdCpdCpdApdApdApdTpdGpdCpdCpdApdCpdCpdCpdTpdTpdCpdApdGpdApdApdTpdTpdCpdGpdCpdApdCpdCpdAp-3'
Molecular Weight: 27523.83
Extinction Coefficient: 840700
Binding Conditions / Buffer
Binding Buffer: 300 mM NaCl, 5 mM MgCl2, 20 mM Tris-HCl at pH 7.6
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Minyong Li, Na Lin, Zhen Huang, Lupei Du, Craig Altier, Hao Fang and Binghe Wang. "Selecting Aptamers for a Glycoprotein through the Incorporation of the Boronic Acid Moiety." J. Am. Chem. Soc., 2008, 130 (38), pp 12636ƒ??12638.
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