Feline Immunodeficiency Virus (F1a) Aptamer Details
ID# 7573
Structure
Chemistry
RNA
Target
Feline Immunodeficiency Virus (FIV)
Category
Protein
Affinity (Kd)
1.9 nM
Length
91
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprGprAprUprAprUprUprUprUprCprUprCprAprGprAprCprCprGprUprAprAprGprUprAprCprCprGprAprAprUprGprUprGprCprUprUprUprUp-Y-rGprGprCprCprGprAprUprUprUprUprUprGprGprCprCprCprCprUprGprCprAprGprUprUprGprCprAprGprCprAprUprCprGprUprGprAprAprCprUprAprGprGprAprUprCprCprGprGprGp-3'
Molecular Weight: 29248.51
Extinction Coefficient: 892700
Binding Conditions / Buffer
Binding Buffer (50 mM Tris-HCl (pH 7.7), 200 mM potassium acetate, and 10 mM dithiothreitol)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Chen, et al. "Inhibitory RNA Ligand to Reverse Transcriptase from Feline Immunodeficiency Virus." Journal of Biochemistry, 35(1996): 6923-6930.
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