Exotoxin A (R14.33) Aptamer Details
ID# 10029
Structure
Chemistry
DNA
Target
Exotoxin A
Category
Protein
Affinity (Kd)
4.2-4.5 µM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdGpdTpdApdCpdCpdGpdTpdCpdTpdGpdApdGpdCpdGpdApdTpdTpdCpdGpdTpdApdCpdCpdApdTpdApdGpdGpdGpdTpdGpdCpdTpdTpdTpdTpdCpdApdApdGpdGpdCpdCpdApdCpdApdCpdGpdTpdTpdApdGpdTpdGpdTpdApdApdGpdCpdCpdApdGpdTpdCpdApdGpdTpdGpdTpdTpdApdApdGpdGpdApdGpdTpdGpdCp-3'
Molecular Weight: 24756 g/mole
Extinction Coefficient: 772800
Binding Conditions / Buffer
100 mM sodium chloride, 20 mM Tris-HCl, and 2 mM magnesium chloride
Binding Temp: Room temperature°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Hong KL, Yancey K, Battistella L, Williams RM, Hickey KM, Bostick CD, Gannett PM, Sooter LJ. Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human Serum. Biomed Res Int. 2015;2015:417641. doi: 10.1155/2015/417641.
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