ex-50.T Aptamer Details
ID# 8143
Structure
Chemistry
2'-F-RNA
Target
Breast cancer exosomes
Category
Other
Affinity (Kd)
0.8 nM
Length
33
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-fUprGpfUprGprGpfCprAprGpfUpfUprAprAprGprAprApfUprAprGprApfUpfCpfUpfUpfCprGpfCpfUprGpfCprGprApfUpfUp-3'
Molecular Weight: 10.652
Extinction Coefficient: 333200
Binding Conditions / Buffer
For Binding Assays on Exosomes:
200 nM of aptamer in 1X PBS
PBS buffer used to wash
For Binding Assays on Cells:
200 nM of aptamer in serum-free medium
Serum-free medium used to wash
Binding Temp: 37°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14,(2008): 1154-1163.
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