Escherichia coli (E. coli) O157:H7 Aptamer Details

ID# 7660

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Structure

Chemistry

Chimeric

Target

Escherichia coli (E. coli) O157:H7

Sequence (3-char table code)

G A C U/T

5'-rGprGprGpfUpfCpfUpfUpfCpfCpfUprGprGprApfCpfUprGpfUpfCprGprAprAprAprApfUpfUpfCprAprGpfUprApfUpfCprGprGprGprAprGprGpfUpfUprApfCprGpfUprApfUpfUpfUprGprGpfUpfUpfUprApfUprAprGprApfUprAprGpfUprAprAp-3'

Molecular Weight: 20656.14

Extinction Coefficient: 662700

Binding Conditions / Buffer

Binding Buffer (30 mM Trisƒ??HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2, 2 mM dithiothreitol, and 1% BSA). Incubated at 37°C for 24 hours.

Binding Temp:

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Lee at al. "In vitro selection of Escherichia coli O157:H7-specific RNA aptamer." Biochemical and Biophysical Research Communications, 417(2012): 214-220.

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