Es2-7bp (17β-estradiol) Aptamer Details
ID# 9522
Structure
Chemistry
DNA
Target
17β-estradiol
Category
Small Organic
Affinity (Kd)
0.6 µM
Length
43
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dGpdGpdApdCpdTpdTpdApdApdGpdGpdTpdApdTpdGpdTpdGpdApdTpdCpdTpdTpdApdGpdTpdTpdGpdTpdApdGpdTpdTpdCpdApdApdGpdTpdCpdCpdTpdTpdApdApdGp
Molecular Weight: 13317.7 g/mole
Extinction Coefficient: 426500
Binding Conditions / Buffer
20 mM HEPES pH 7.4,
100 mM NaCl, 8 mM MgCl2
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Shi, L., Jin, Y., & Liu, J. (2024). Intramolecular aptamer switches. The Analyst, 149(3), 745–750. https://doi.org/10.1039/d3an02022c
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